ESC9: Correlative microscopy: from cells to single molecules

Description of the course:

MOSBRI ESC9 will take place as part of a larger annual workshop, the “9^th NIC@IIT and MOSBRI Advanced Microscopy practical workshop“, organised by the Nikon Imaging Centre and the Istituto Italiano di Tecnologia in Genoa.

The course will cover topics such as: sample preparation, atomic force microscopy and spectroscopy, confocal microscopy, image scanning microscopy (ISM); STED; STORM, d-STORM, live cell imaging, 2PEFM, FLIM, FCS, fast volumetric imaging, expansion microscopy.

During the course the participants will learn about the fundamental background and operational procedures of the techniques and hands-on practice with the instruments will be performed. Attention will be given to sample preparation, set-up operation, data gathering and data analysis.

Outline of the course:

The course will start in the afternoon of Monday 2^nd of December and end at lunchtime on Friday 6^th of December and will combine both theoretical and practical content. On the Monday there will be Keynote lectures given by invited speakers, with further lectures on Tuesday morning and then practical sessions for the rest of the week.

The hands-on sessions will be organized to give basic knowledge on the topics and the available instruments. The attendees will be divided into groups of a maximum of five people, and each group will have the opportunity to work on every workstation.

It will be possible to bring your own samples for use at the workshop, if you would like to do so please include details of the samples in the application form (below).

Day 1

Welcome and registration

Keynote Lectures

Day 2

Morning: Lectures
Afternoon: Hands-on practical session

Days 3-5

Hands-on practical sessions, ending at lunch time on the Friday.

Other details:

The course is aimed at researchers with some experience in protein biophysics who wish to acquire or reinforce new scientific and technical skills: graduate and PhD students, postdoctoral fellows, early career scientists, technicians, and core facility staff.

The workshop will be limited to around 40 participants, in order to have no more than 5 people per group for the practical sessions. Details of how to register can be found on the workshop’s website https://mix.iit.it/events/iit-nikon-imaging-center/9th-nic-iit-and-mosbri.

MOSBRI supports attending this workshop, for participants coming from places where such training is not available locally or in close proximity. 10 selected participants will receive supporting bursaries to attend the course. The bursary will include the registration and 4 nights of accommodation with breakfast (accommodation will be booked by the organizers). Successful applicants will be informed of the eligible expenses when they receive a formal acceptance letter.

Those applying for support through MOSBRI should also register according to the instructions given on the workshop website.

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Application for a bursary

The number of MOSBRI supported places for this workshop is limited to 10 and therefore any interested parties will need to submit an application for consideration to receive a bursary.

Who is eligible to apply:

Anyone with a workplace/host institution in one of the EU member or associated states may apply for a bursary for this course (this includes people from the UK). Applicants from industry are also welcome. It is expected that applicants have at least a basic knowledge of protein biophysics and that the topic of the course is applicable to their area of research/work. Applications from scientists in the early-stage of their career are particularly encouraged and we will strive to obtain a gender balance of the participants in the course.

How to apply:

When to apply:

It is now possible to apply for bursary. Completed application forms, along with any supporting documentation should be sent to

Application deadline:

The deadline for submission of an application for MOSBRI support to participate in this course is the: 15^th October 2024.