ESC9: Correlative microscopy: from cells to single molecules

MOSBRI ESC9 will take place as part of a larger annual workshop, the “9th NIC@IIT and MOSBRI Advanced Microscopy practical workshop“, organised by the Nikon Imaging Centre and the Istituto Italiano di Tecnologia in Genoa.

The course will cover topics such as: sample preparation, atomic force microscopy and spectroscopy, confocal microscopy, image scanning microscopy (ISM); STED; STORM, d-STORM, live cell imaging, 2PEFM, FLIM, FCS, fast volumetric imaging, expansion microscopy.

During the course the participants will learn about the fundamental background and operational procedures of the techniques and hands-on practice with the instruments will be performed. Attention will be given to sample preparation, set-up operation, data gathering and data analysis.

Read more about this course and apply for support here.

Message from the cell – Workshop

Understanding how the cellular environment affects biomolecule structures, dynamics and their interactions.

We are pleased to announce the second edition of “MESSAGE FROM THE CELL ”, which will be held in Marseille (France) on the 21st and 22nd November 2024.

The workshop will focus on “cellular structural biology” and on complementary techniques.

Confirmed Keynote speakers:

Jean Pierre SIMORRE, FR
Sharon RUTHSTEIN, IL
Shahar Sukenik , USA
Frederic TARAN, FR

Scientific Committee:

Elisabetta MILEO, François Xavier THEILLET, Latifa ELANTAK , Hélène LAUNAY, Alessio BONUCCI, Valérie BELLE

More information about the programme and how to register can be found at the workshop webpage.

Towards data FAIRness in molecular biophysics

t is increasingly difficult for biophysical researches to keep up with what has been measured and how the measurement was performed. This is partly due to accumulation of data over time and partly due to requirements to Methods and Material sections varying substantially depending on the journal. The next hurdle is reached when researchers want to reanalyse data using alternative models or want to conduct large scale meta-analysis. Both of these goals would be greatly aided by access to the raw measurement data and results in a searchable form, especially if sufficient metadata is present to undertake the analysis in a fully or semi-automatic fashion. Currently, it is still not commonplace to deposit biophysical data, and even less likely to deposit it in a structured form. This is of course where data management and annotation comes in, and due to the amount of data present, it is becoming an increasingly critical task.

At this conference we’re bringing together researchers from various repositories and databases, as well as users, so they can present the latest developments in the resources themselves and the developments in how the resources are used.

The focus will in particular be on interoperability and integrative approaches in biophysics.

Read more about the meeting and register here.

Biophysical Methods for Protein Interactions Workshop

September 23-27, 2024, Warsaw, Poland

Workshop Description

This workshop will review the strategies for using hydrodynamic and thermodynamic analytical methods to characterize biological macromolecules and their interactions in solution. We will discuss theoretical and practical aspects of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and mass photometry (MP).

This week-long workshop will start on Monday, September 23rd, at 8:30 AM and end on Friday, September 27th, at 5 PM. We will provide an introduction to the experimental aspects of AUC, DLS, and ITC, including the operation of the instruments, choice of buffers, and the experimental design appropriate for the system under study. This workshop has a strong focus on AUC, and topics such as the assembly of the AUC cells, the operation of the XLA/I analytical ultracentrifuge, as well as advanced applications of AUC to the study of interacting and non-interacting systems will be discussed in detail. Over the course of the workshop, the theory, data analysis, and experimental strategy of other biophysical methods will also be discussed. These lectures will be accompanied by practical computer exercises with the SEDFIT and SEDPHAT data analysis software.

Participants’ presentations of their systems of interest and an informal discussion of their data will be an integral part of the workshop.

Registration is now open

Registration deadline: June 30, 2024.

Target Audience
This workshop is aimed at researchers with at least basic experience in the applications of AUC, DLS, ITC, SPR/BLI, MP, and/or fluorescence techniques for the characterization of biomacromolecules.

Faculty

  • Chad Brautigam, PhD
    Department of Biophysics, UTSW Medical Center, Dallas, Texas
  • Rodolfo Ghirlando, PhD
    Laboratory of Molecular Biology, NIDDK, NIH
  • Grzegorz Piszczek, PhD
    Biophysics Core, NHLBI, NIH
  • Peter Schuck, PhD
    Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH
  • Huaying Zhao, PhD
    Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH

The program of the workshop will be identical to the program of the workshop taking place in May in National Institutes of Health, Bethesda, MD, USA.

Workshop Organizing Committee
Roman Szczepanowski, IIMCB
Beata Wielgus-Kutrowska, University of Warsaw
Krzysztof Skowronek, IIMCB

 

MOSBRI: Molecular Biophysics to understand life and disease

MOSBRI Special Session at FEBS2024, 15:30-16:00, 1st July 2024, Milan.

MOSBRI: Molecular Biophysics to understand life and disease.
15:30 – 15:45 Patrick England, Institut Pasteur, Paris, France.
15:45 – 16:00 Francesca Cutruzzolà, Sapienza University, Rome, Italy.

The pan-European MOlecular-Scale Biophysics Research Infrastructure MOSBRI (mosbri.eu) was launched in 2021 to provide free-of-charge trans-national access (TNA) to cutting-edge instrumentation and expertise in molecular biophysics through an integrated consortium including partners from 11 European countries, thus enabling excellent life science research. In this special session, we will highlight success stories selected from the more than 150 TNA projects accepted to date from applicants worldwide, and showcase some of the networking activities (international conferences, courses and training schools) and joint research activities that MOSBRI has spearheaded.

 

FEBS2024 programme

48th FEBS Congress, Milan

The 48th FEBS Congress – ‘Mining biochemistry for human health and well-being’ – will be hosted in Milano, Italy, from the 29th of June 29 to the 3rd of July 2024, for scientists from all over Europe and other parts of the world. The Italian Society of Biochemistry and Molecular Biology (SIB), founded in 1951, will be the host Society of the event.

The 48th FEBS Congress will present a broad-spectrum and ground-breaking scientific program, completed in a fruitful collaboration with the journals of FEBS and representatives from FEBS Constituent Societies. The focus on human health and well-being, through molecular and cellular science approaches, reflects a key social issue and a specific commitment of SIB and the whole biochemical community in a time of ongoing global transitions. Besides important research topics following the latest and emerging developments in the biochemical sciences, the Congress activities will include dedicated education-oriented sessions, symposia organized by young biochemists, and biochemistry-related economic discussions. There will also be opportunities for presentations from industry of the latest advances in commercial instrumentation.

Read more about the congress and register here.

ESAB 2024: European South Atlantic Biophysics Congress

the European South Atlantic Biophysics Congress that will take place in Donostia-San Sebastian (Basque Country), on June 5th-7th , 2024. The event will include a Satellite Workshop on June 4th-5th, 2024, focused on early-stage researchers, with a total attendance of 50 students.
The meeting is being organized as a joint effort by the Spanish Biophysical Society (SBE), the French Biophysical Society (SBF) and the Portuguese Biophysical Societies (SPBf), to gather scientists for presenting their most exciting biophysical research work.

The main Local Organizer, CIC biomaGUNE, is managing the preparation of the ESAB 2024 Congress in collaboration with local institutions such as Donostia International Physics Center, CIC bioGUNE, Instituto Biofísica and the University of Basque Country UPV/EHU.

This congress is the first event of a regional agreement between the three societies in order to organize periodic conferences promoting biophysics, especially amongst young scientist in the South-West of Europe. This congress will be an excellent opportunity for researchers to meet and discuss opportunities to collaborate in a genuinely multidisciplinary environment in the field of Biophysics. We hope that the participation of three societies will broaden the networking activities and the dissemination of knowledge in biophysics.

Visit the website to find out more about the congress and to register.

https://www.biophysicssansebastian2024.com/

Course on Protein Quality Control

This course is organized by Prof. André Matagne, Laboratory of Enzymology and Protein Folding, Centre for Protein Engineering, University of Liège, Belgium, on behalf of the F.R.S.-FNRS Graduate School “Structure and Function of Biological Macromolecules, Bioinformatics and Modelling” and the Belgian Biophysical Society.

Purified proteins stand as a central focus in numerous research laboratories, where they are used for a wide spectrum of applications ranging from 3D structural analysis, through X-ray, cryoEM, and NMR studies, to in-depth investigations into interactions (protein-protein, protein-ligand, or protein-nucleic acid) and cell biology experiments. Furthermore, these proteins play a crucial role in characterizing biological (e.g. enzymatic) activities. Beyond the realm of academic research, purified proteins serve as efficient tools in the therapeutic and diagnostic landscape, finding applications in various pharmaceutical industries. To ensure the reliability of experimental data in these studies, stringent protein quality controls are imperative. In 2021, the collaborative efforts of the ARBRE-MOBIEU and P4EU networks resulted in the publication of comprehensive guidelines for evaluating purified proteins in life sciences and medical trials (de Marco et al., 2021; Berrow et al., 2021). The objective of this course is to familiarize participants with these guidelines for protein quality control and to provide an overview of the technologies essential for enhancing the accuracy and reproducibility of data obtained with purified proteins. Throughout the course, participants will have access to the theoretical foundations of these technologies and will also gain hands-on experience with them, ensuring a comprehensive learning experience.

Read more about the course and apply here:
https://www.cip.uliege.be/cms/c_12068241/en/course-on-protein-quality-control

The deadline for applications is: 15th March 2024.